False unknows: Annotated as unknown or general but function is known and published
- failure in capturing literature
- Not captured in any database
- Captured in some databases but not others
- Naming problem (inconsistent naming of same entities)
- Failure in progagating to orthologs ( captured for one member of a family but not propagated to others)
False knowns: Annotated as precise but wrong ( should be general or another annotation) or incomplete
- Naming problem (inconsistent naming of same entities)
- Fusions (only one of the functional roles is called)
- Multiple functions (Moonlighting or Promiscuity only one of the functional call is called)
- Curation mistake ( the data was incorrectly captured by biocurator)
- Experimental mistake, poor science or finding has been refuted by other studies (the published data does not make sense or is easily challenged by an expert , or new data shows it was wrong)
- Over-annotation of paralogs